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correct-3d-drift.md

correct-3d-drift.md

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正确的 3D 漂移

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该斐济菜单命令由脚本Correct_3D_Drift.py实现。

该脚本要求当前打开活动的图像是由随时间变化的 2D 或 3D 卷组成的超堆栈（或虚拟超堆栈）。该脚本使用§§2§§§的相反相关实现来注册各自的时间点。
实施后，将出现一个对话框，要求用户选择用于注册的颜色通道。 3.如果系统内存有限，则用户可以选择使用虚拟堆栈来获取结果。 4.如果选择了虚拟堆栈，则需要选择保存堆栈的文件夹。 5.经过一些处理（有详细信息条）后，将打开生成的注册卷。如果选择了堆栈虚拟，则注册卷将保存在所选目标文件夹中已。

该基层最初是由Albert Cardona和Robert Bryson-Richardson在参加Gabriel G. Martins组织的Developmental Imaging EMBO course时编写的。

出版

Parslow A, Cardona A and Bryson-Richardson RJ (2014) Sample drift correction following 4D confocal time-lapse imaging. J Vis Exp. 2014 Apr 12;(86). doi: 10.3791/51086。