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历史
该插件由 Kota Miura 和 Jens Rietdorf 提供，完整文档可在 here 中获取。
引文
doi:10.12688/f1000research.27171.1
详细信息
该插件包含透明不同的方法来修复由于光遮蔽引起的强度衰减。它们都适用于 2D 或 3D 时间序列。对于 3D 时间序列，应适当设置图像属性。如果您不确定，请通过[Image → Properties]检查您的标题图像。
- 简单区分法：
- Plugin version of Jens Rietdorf’s macro, extended further with 3D time series
- 指数法：
- Similar to the description on the T-functions page. Additionally, this plugin also works with 3D time series.
- MBF ImageJ suggests to use “Exponential” equation for fitting, whereas this plugin uses “Exponential with Offset”
- 直方图匹配方法：
- A brand-new method for bleach correction.
- This algorithm first samples the histogram of initial frame, and for the successive frames, histograms are matched to the first frame. This avoids the increase in noise in the latter part of the sequence which is a problem in the above two methods.
- This method does much better restoration of bleaching sequence for segmentation but not appropriate for intensity quantification.
- See the blog entry, a bit more detail on this issue
视频教程
请参见此处：Youtube
